Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Metabolism Kits > Glutamate Dehydrogenase Detection Kit

Glutamate Dehydrogenase Detection Kit

"GDH" Related Products.
Product Overview:
Glutamate Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
100 x 1 assay
Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
Functional Studies
Store kit at -20°C, protect from light. Warm the Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Read the entire protocol before performing the assay.
Kit Components:
Components Identifier 100 testsGDH Assay Buffer WM 1 x 25mlGDH Developer Red 1 vialGDH Positive Control Green 1 vialGlutamate Blue 1 x 1mlNADH Yellow 1 vial
Compatible Sample Types:
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
>0.01 mU/well

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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