Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Glutathione Reductase Assay Kit

Glutathione Reductase Assay Kit

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Cat.No.:
Kit-0391
Product Overview:
Glutathione Reductase Assay Kit is used for measuring GR activity.
Description:
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. The Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with5, 5"-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store kit at -20°C, protect from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
Kit Components:
GR Assay Buffer 100 ml3% H2O2 1 mlCatalase (lyophilized) 1 vialTNB Standard (lyophilized) 1 vialDTNB (lyophilized) 1 vialNADPH-GNERAT (lyophilized) 2 vialsGSSG (lyophilized) 1 vialGR Positive Control (10 mU; lyophilized) 1 vial
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Erythrocyte Lysate, Plasma, Serum, Tissue Culture Supernatant, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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