Glutathione Reductase Assay Kit is used for measuring GR activity.
Glutathione Reductase (GR, EC 18.104.22.168) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. The Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with5, 5"-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
For research use only (RUO)
Store kit at -20°C, protect from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
GR Assay Buffer 100 ml3% H2O2 1 mlCatalase (lyophilized) 1 vialTNB Standard (lyophilized) 1 vialDTNB (lyophilized) 1 vialNADPH-GNERAT (lyophilized) 2 vialsGSSG (lyophilized) 1 vialGR Positive Control (10 mU; lyophilized) 1 vial
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Erythrocyte Lysate, Plasma, Serum, Tissue Culture Supernatant, Urine