"GR" Related Products

Glutathione Reductase Assay Kit

Cat.No.: Kit-0391
Product Overview: Glutathione Reductase Assay Kit is used for measuring GR activity.
Description: Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. The Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with5, 5"-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
Target Species: Mammals
Usage: For research use only (RUO)
Storage: Store kit at -20°C, protect from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
Kit Components: GR Assay Buffer 100 ml3% H2O2 1 mlCatalase (lyophilized) 1 vialTNB Standard (lyophilized) 1 vialDTNB (lyophilized) 1 vialNADPH-GNERAT (lyophilized) 2 vialsGSSG (lyophilized) 1 vialGR Positive Control (10 mU; lyophilized) 1 vial
Detection method: Colorimetric
Compatible Sample Types: Biological Fluid, Cell Culture Supernatant, Erythrocyte Lysate, Plasma, Serum, Tissue Culture Supernatant, Urine

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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