Glycine Fluorometric Assay Kit

• Cat.No.: Kit-2141
• Product Overview: Glycine Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentration of glycine in a variety of biological fluids. The principle of the assay is based on the oxidation of glycine producing a fluorophore (Ex/Em = 535/587 nm) with a stable signal, which is directly proportional to the amount of GLY in the sample. The assay is specific and other standard and non-standard amino acids do not interfere with the assay. The assay can detect as little as 1 µM of Glycine in a variety of samples.

Specification

• Size: 100 assays
• Applications: Estimation of glycine in various biological samples
• Storage: Store kit at -20°C, protected from light. Briefly spin small vials prior to opening. Read entire protocol before performing the assay.GLY Assay Buffer: Store at -20°C or 4°C. Bring to room temperature (RT) before use.GLY Probe: Light sensitive. Store at -20°C. Bring to RT before use.GLY Enzyme Mix: Reconstitute with 55 µl GLY Assay Buffer containing 10% molecular biology grade glycerol (not provided). MakeGLY Assay Buffer containing 10% glycerol by adding 10 µl of 100% Glycerol to 90 µl of GLY Assay Buffer, vortex for 30 sec. Aliquotand store at -20°C. Light sensitive, protect from light. Freeze/thaw should be limited to two times. Keep on ice during use.GLY Developer: Reconstitute with 220 µl of GLY Assay Buffer. Aliquot and store at -20°C. Freeze/thaw should be limited to one time.Keep on ice during use.Glycine Standard: Reconstitute with 100 µl of dH2O to generate 100 mM Glycine Standard. Dissolve completely. Store at -20°C. Usewithin 2 months.