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Home > Products > Assay Kits > Activity Kits > HAT Activity Fluorometric Assay Kit

HAT Activity Fluorometric Assay Kit

"HAT" Related Products.
Product Overview:
Simple, rapid & convenientThe assay can detect HAT Activity as low as 6 mU in a variety of samples
100 assays
Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision"s HAT Activity Assay Kit utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products-acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.
Measurement of HAT activity in Nuclear ExtractsMeasurement of HAT activity of purified enzyme preparations
Target Species:
For Research Use Only! Not For Use in Humans
Kit Components:
• HAT Assay Buffer• Acetyl CoA (Lyophilized) • H3 Peptide (Lyophilized)• Substrate Mix (Lyophilized)• Developer• PicoProbe• CoA Standard (Lyophilized)• Positive Control (HeLa Nuclear Extract)
Detection method:
Fluorescence (Ex/Em = 535/587 nm)
Compatible Sample Types:
• Nuclear extracts from cells and tissue• Recombinant enzyme

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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