Histone Acetyltransferase Activity Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Reacts with: all Mammals
Store kit at -80°C.
Components Identifier 100 tests2X HAT Assay Buffer Amber 1 x 7.5mlHAT Reconstitution Buffer Clear 1 x 1.8mlHAT Substrate I Blue 1 vialHAT Substrate II Purple 1 vialNADH Generating Enzyme Green 1 x 800µlNuclear Extract (4 mg/ml ) Red 1 x 50µl
Compatible Sample Types:
Tissue Extracts, Cell Lysate