Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Histone Acetyltransferase Activity Colorimetric Assay Kit

Histone Acetyltransferase Activity Colorimetric Assay Kit

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Cat.No.:
Kit-0410
Product Overview:
Histone Acetyltransferase Activity Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Size:
100 x 1 assay
Description:
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Applications:
Functional Studies
Target Species:
Reacts with: all Mammals
Storage:
Store kit at -80°C.
Kit Components:
Components Identifier 100 tests2X HAT Assay Buffer Amber 1 x 7.5mlHAT Reconstitution Buffer Clear 1 x 1.8mlHAT Substrate I Blue 1 vialHAT Substrate II Purple 1 vialNADH Generating Enzyme Green 1 x 800µlNuclear Extract (4 mg/ml ) Red 1 x 50µl
Compatible Sample Types:
Tissue Extracts, Cell Lysate


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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