"HAT" Related Products

Histone Acetyltransferase Activity Colorimetric Assay Kit

Cat.No.: Kit-0410
Product Overview: Histone Acetyltransferase Activity Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Size: 100 x 1 assay
Description: Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Applications: Functional Studies
Target Species: Reacts with: all Mammals
Storage: Store kit at -80°C.
Kit Components: Components Identifier 100 tests2X HAT Assay Buffer Amber 1 x 7.5mlHAT Reconstitution Buffer Clear 1 x 1.8mlHAT Substrate I Blue 1 vialHAT Substrate II Purple 1 vialNADH Generating Enzyme Green 1 x 800µlNuclear Extract (4 mg/ml ) Red 1 x 50µl
Compatible Sample Types: Tissue Extracts, Cell Lysate

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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