Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Epigenetic Kits > Histone Demethylase LSD1 Activity/Inhibition Assay Kit

Histone Demethylase LSD1 Activity/Inhibition Assay Kit

"Histone Demethylase" Related Products.
Product Overview:
Histone Demethylase LSD1 Activity/Inhibition Assay Kit is use for measuring cellular LSD1 activity/inhibition.
Lysine histone methylation is one of the most robust epigenetic marks and is essential for the regulation of multiple cellular processes. The methylation of H3-K4 seems to be of particular significance, as it is associated with active regions of the genome. H3-K4 methylation was considered irreversible, until the identification of a large number of histone demethylases indicated that demethylation events play an important role in histone modification dynamics. So far, at least 2 classes of H3-K4 specific histone demethylase, LSD1 and JARIDs have been identified. LSD1 can remove di- and mono-methylation from H3-K4 by using an amine oxidase reaction. LSD1 histone demethylases are found to be involved in some pathological processes such as cancer progression. Inhibition of the enzymes may lead to re-methylation of H3-K4 and silencing of H3-K4 enriched active genes. Currently there are few methods available for measuring activity/inhibition of LSD1 using a variety of cells/tissues.
The Histone Demethylase LSD1 Activity/Inhibition Assay Kit is very suitable for measuring histone demethylase LSD1 activity/inhibition from a broad range of species including mammalian cell/ tissues, plants and bacteria.
For research use only (RUO)
Upon receipt: (1) Store HG3, HG4, and HG6 at –20°C away from light; (2) Store all other components (HG1, HG2, HG5, HG7, and 8-well Assay Strip) at 4°C away from light. The kit is stable for up to 6 months when stored properly.Note: Check if wash buffer, HG1, contains salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffer until the salts are re-dissolved.
Kit Components:
HG1 (10X Wash Buffer) 30 mLHG2 (LSD1 Assay Buffer) 4 mLHG3 (LSD1 Substrate)* 100 μLHG4 (LSD1 Assay Standard, 20 μg/mL)* 50 μLHG5 (Capture Antibody 1000 μg/mL)* 10 μLHG6 (Detection Antibody 200 μg/mL)* 16 μLHG7 (Fluoro-developer) 12 mL8-well Assay Strip (with Frame) 12 strips* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
Features & Benefits:
Fast procedure, which can be finished within 3 hours.Innovative fluorescent assay without the need for radioactivity, extraction, or chromatography.Direct measurement of LSD1 activity and no interference by thiol-containing chemicals such as DTT, GSH and 2-mercaptoethanol.Both cell/tissue extracts and purified LSD1 can be used, which allows the detection of inhibitory effects of LSD1 inhibitor in vivo and in vitro.Strip microplate format makes the assay flexible: manual or high throughput analysis.Simple, reliable, and consistent assay conditions.

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.