Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Epigenetic Kits > JMJD2A Tudor Domains TR-FRET Assay Kit

JMJD2A Tudor Domains TR-FRET Assay Kit

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Cat.No.:
Kit-1965
Size:
9600 wells
Description:
The posttranslational modification (PTM) of chromatin plays a crucial role in the regulation of gene transcription, DNA repair and many other cellular processes. Collectively, the combinatorial array of chromatin PTMs forms the foundation of the "Histone Code". This code is interpreted by a large family of protein motifs that selectively recognize and bind to a particular chromatin PTM. Commonly found in transcription factors and other regulatory proteins, these reader domains localize their proteins to chromatin bearing the appropriate PTM(s). Additionally, a given protein will often have multiple reader domains located adjacent to each other, potentially providing additional levels of specificity to the system. The JMJD2A Tudor Domains TR-FRET Assay Kit is a homogeneous, TR-FRET assay method amenable to rapid characterization of Tudor domain inhibitors in a high-throughput format. This screening assay is robust, displaying a Z" >0.6, and is designed to assist in the early-stage discovery and development of chromatin reader domain inhibitors.
Storage:
-80°C
Kit Components:
JMJD2A Tudor Domains Europium Chelate: 1 vial/420 wells; 5 vials/ 420 wells; 5 vials/ 2,100 wells; -80°CJMJD2A Tudor Domains Ligand/APC Acceptor Mixture: 1 vial/420 wells; 5 vials/ 420 wells; 5 vials/ 2,100 wells; -80°C TR-FRET Assay Buffer 2 (10X): 1 vial/2 ml; 1 vial/10 ml; 5 vials/10 ml; -20°C JMJD2A Tudor Domains Positive Control: 1 vial/2.5 nmol; 1 vial/12.5 nmol; 5 vials/12.5 nmol; -20°C384-Well Solid Plate (low volume; black): 1 plate; 5 plates; 25 plates; Room temperature Foil Plate Covers: 1 cover; 5 covers; 25 covers; Room temperature


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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