JMJD2E, also known as KDM4DL (lysine (K)-specific demethylase 4D-like) is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.
The JMJD2E Homogeneous Assay Kit is designed to measure activity of JMJD2E for screening and profiling applications, using AlphaLISA technology. The key to the JMJD2E Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2E enzyme is incubated with the biotinylated substrate for two hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alphacounts.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
JMJD2E: 40 µg; -80°C Primary antibody 5: 2 x 12.5 μl; -80°C Biotinylated histone H3 peptide substrate: 500 rxns; -80°C 4x HDM Assay Buffer 3: 2 ml; -20°C 4x HDM Incomplete assay buffer: 1 ml; -20°C 4x Detection buffer: 2 ml; -20°C