Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > JMJD3 (KDM6B) Homogeneous Assay Kit
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Product Overview:
JMJD3 is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3.
384 reactions
The JMJD3 Homogeneous Assay Kit is designed to measure activity of the JMJD3 for screening and profiling applications, using AlphaLISA technology. The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
JMJD3: 40 µg; -80°C Primary antibody 6: 40 µl; -80°C Biotinylated histone H3 peptide substrate: 500 rxns; -80°C 4x HDM Assay Buffer 3: 2 ml; -20°C 4x HDM Incomplete assay buffer: 1 ml; -20°C 4x Detection buffer: 2 ml; -20°C

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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