"L-Lactate" Related Products

L-Lactate Assay Kit

Cat.No.: Kit-0518
Product Overview: L-Lactate Assay Kit is a quantitative fluorimetric determination of L-lactate at FL530/585 nm.
Description: LACTATE is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. L-Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.
Applications: Direct Assays: L-lactate in serum, plasma, urine, cell media samples and other biological samples.
Usage: For research use only (RUO)
Storage: Store all reagents at -20°C. Shelf life: 6 months after receipt.
Kit Components: Assay Buffer 10 mLNAD Solution 1 mLProbe 750 μLEnzyme A 120 μLEnzyme B 120 μLStandard 1 mL
Detection method: Fluorimetric
Compatible Sample Types: Culture Medium, Plasma, Serum, Urine
Features & Benefits: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM L-lactate in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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