Assay kit useful for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in human plasma. LCAT mediates the formation of cholesteryl esters in human plasma by transferring an acyl chain from the sn-2 position of phosphatidylcholine to cholesterol.
The Calbiochem LCAT Activity Assay Kit, Fluorometric is intended for the quantitative measurement of lecithin:cholesterol acyltransferase activity (LCAT) in normal human plasma. This kit allows the quantitation of fold of increase or decrease of enzymatic activity among samples, this kit also allows the quantitation of the % change of 470/390 ratio with time, which can be used to define LCAT enzymatic activity. This kit does not allow the measurement of substrate concentration or the amount of hydrolyzed substrate.
Storage: Multiple storage requirements Storage Conditions: Upon arrival store the LCAT Substrate at -20°C and the READ Reagent at 4°C.Protect from Light: Protect from light Do not freeze: No
There are sufficient reagents for 100 tests.LCAT Substrate: 1 vial, 100 µlREAD Reagent: 1 vial, 350 µl
Compatible Sample Types: