Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Lactose Assay Kit

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Cat.No.:
Kit-0500
Product Overview:
Lactose Assay Kit is a quantitative colorimetric lactose determination.
Description:
LACTOSE (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. Lactose Assay Kit uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
Applications:
Assays of lactose in milk and other biological samples.Drug Discovery/Pharmacology: effects of drugs on lactose metabolism.Food and Beverages: lactose in food and beverages products.
Usage:
For research use only (RUO)
Storage:
Store all components at -20°C. Shelf life of 12 months after receipt.
Kit Components:
Assay Buffer 10 mLEnzyme Mix DriedLactase DriedDye Reagent 120 µLStandard (20 mM Lactose) 1 mL
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Sample, Milk
Features & Benefits:
Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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