Lactose Assay Kit is a quantitative colorimetric lactose determination.
LACTOSE (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. Lactose Assay Kit uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
Assays of lactose in milk and other biological samples.Drug Discovery/Pharmacology: effects of drugs on lactose metabolism.Food and Beverages: lactose in food and beverages products.
For research use only (RUO)
Store all components at -20°C. Shelf life of 12 months after receipt.
Assay Buffer 10 mLEnzyme Mix DriedLactase DriedDye Reagent 120 µLStandard (20 mM Lactose) 1 mL
Compatible Sample Types:
Biological Sample, Milk
Features & Benefits:
Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays.