Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

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Cat.No.:
Kit-0542
Product Overview:
Simple procedure
Fast and convenient
Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples
Description:
Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Applications:
The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Usage:
For Research Use Only! Not For Use in Humans.
Storage:
-20°C
Size:
100 assays
Kit Components:
• MDA Lysis Buffer
• Phosphotungstic Acid Solution
• BHT (100X)
• TBA
• MDA Standard (4.17M)
Target Species:
All
Detection method:
Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)
Compatible Sample Types:
Cell and Tissue culture supernatants, plasma and other biological fluids (optimized by end user).


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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