Maleimide Assay Kit (Green Fluorescence) is a one-step fluorometric method for the quantification of maleimide groups in a variety of molecules such as proteins.
Sensitive assays of maleimide and thiol groups are required for the efficient conjugation of proteins that are expensive and available only in small amounts. A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules. There are few reagents or assay kits available for quantifying the number of maleimide groups that are introduced into the first protein. All the commercial kits have tedious protocols.
Maleimide Assay Kit (Green Fluorescence) uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide.
For research use only (RUO)
Store the kit at -20°C and avoid from light.
Component A: Maleimide Green 1 vialComponent B: Reaction Buffer 500 μLComponent C: Assay Buffer 25 mLComponent D: N-ethylmaleimide Standard (10 mM) 50 μLComponent E: DMSO 200 μL
Features & Benefits:
Broad Application: Can be used for quantifying maleimide group in a variety of molecules such as proteins.Sensitivity: Detects as low as 10 picomoles of maleimide.Continuous: Easily adapted to automation without a separation step.Convenient: Formulated to have minimal hands-on time. No wash is required.Non-Radioactive: No special requirements for waste treatment.