Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Other Kits > Maleimide Rapid Assay Kit

Maleimide Rapid Assay Kit

   Share
"Maleimide" Related Products.
Cat.No.:
Kit-0535
Product Overview:
Maleimide Assay Kit (Rapid) is a quantitative assay for the quantification of maleimide groups.
Description:
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules. A key challenge in maleimide crosslinking technology has been the development of reliable and reproducible methods for quantitation the amount of maleimide linked to a single protein or antibody. Maleimides can be directly assayed spectrophotometrically at 302 nm. However, the small extinction coefficient of 620 M-1cm-1 renders this assay insensitive, and the assay is further complicated by the protein absorbance at the same wavelength. There are a few methods available for quantitating the number of maleimide groups by indirect ways. And in many cases, the maleimide ratio is only estimated by experience.
Applications:
Maleimide Assay Kit (Rapid) provides an accurate method to quantify maleimide group by our maleimide sensor Maleimide Blue with the maximum absorbance at ~780nm.
Usage:
For research use only (RUO)
Storage:
Upon receipt, store Maleimide Blue (Component A) the kit at 2-6°C, kept from light and moisture. When stored properly, the kit components should be stable for six months.Note 1: Do not freeze Spin Column (Component C).Note 2: Warm all the components before run the required assays. 50 to 100 μg maleimide-linked antibody or protein sample is needed for determining the amount of maleimide.
Kit Components:
Component A: Maleimide Blue (One vial is for 50 ~100 μg maleimide-linked antibody or protein) 2 vialsComponent B: Assay Buffer 15 mLComponent C: Spin Column 2 columnsComponent D: Washing Tube 2 mLComponent E: Collecting Tube 1.5 mL
Detection method:
Colorimetric
Compatible Sample Types:
Polymers, Proteins


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.