Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Other Kits > Mammalian Chromatin Extraction Kit
"ChIP" Related Products.
Product Overview:
Mammalian Chromatin Extraction Kit is used for isolating chromatin or DNA-protein complex from mammalian cells or tissues.
Chromatin immunoprecipitaton (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, the ChIP method with specific antibodies directly against various transcriptional factors is widely demanded. For performing ChIP, chromatin or DNA-protein complex in cells or tissues should be first isolated.
The Mammalian Chromatin Extraction Kit is suitable for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Target Species:
For research use only (RUO)
Upon receipt: (1) Protease Inhibitor cocktails at 4°C; (2) Store remaining components at room temperature.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: Check if any buffers contain salt precipitates before use. If so, shake the buffer until the salts are re-dissolve.
Kit Components:
10X Lysis Buffer 11 mlExtraction Buffer 11 mlChromatin Buffer 11 mlProtease Inhibitor Cocktails (1000X)* 110 µl* Spin the solution down to the bottom prior to use.
Compatible Sample Types:
Cell, Tissue
Features & Benefits:
Extremely fast procedure: the entire procedure from cell/tissue sample to ready-to-use chromatin is less than 60 minutes.Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues.Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, etc.

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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