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Home > Products > Assay Kits > Cellular Kits > Multi-Drug Resistance Assay Kit (Green)

Multi-Drug Resistance Assay Kit (Green)

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Cat.No.:
Kit-0546
Product Overview:
Multi-Drug Resistance Assay Kit use a fluorescent MDR indicator for assaying two ATP-dependent drug-efflux pump activities.
Description:
Multi Drug Resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP- dependent drug-efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug- resistance-associated protein (MRP1) are members of the ABC transporter family. Our Multi-Drug Resistance Assay Kit (Green) uses a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporters, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Multi-Drug Resistance Assay Kit (Green) are ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C and avoid exposure to light.
Kit Components:
Component A: MDR Sensor 1 vialComponent B: DMSO 1 vial (100 μl)Component C: Assay Buffer 1 bottle (10 ml)
Detection method:
490/525 nm
Compatible Sample Types:
Cell Culture


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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