Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
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Neutrophil/Monocyte Respiratory Burst Assay Kit

"Neutrophil" Related Products.
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Respiratory burst, or the rapid generation of reactive oxygen species from immune cells, is crucial for the destruction of invading microorganisms by phagocytes. During this process, professional phagocytes convert molecular oxygen to superoxide anions through the action of NADPH oxidase, which is then converted to reactive oxygen species with potent antimicrobial activity. The Neutrophil/ Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.
Kit Components:
Dihydrorhodamine 123 Assay Reagent: 1 vial/50 µl; -20°C PMA (1 mM) Assay Reagent: 1 vial/50 µl; -20°CRBC Lysis Buffer (10X): 1 vial/10 ml; 4°C Bovine Serum Albumin Assay Reagent: 1 vial/5 g; 4°CCalcium Chloride (1 M) Assay Reagent: 1 vial/1 ml; RT

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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