"NO" Related Products

Nitric Oxide Fluorimetric Detection Kit (Red)

Cat.No.: Kit-2046
Product Overview: Nitrixyte probes are developed and used in our kit as an excellent replacement for DAF-2 for the detection and imaging of free NO in cells. Compared to the commonly used DAF-2 probe, Nitrixyte probes have better photo stability and enhanced cell permeability.This particular kit uses Nitrixyte Red that can react with NO to generate a bright red fluorescent product that has spectral properties similar to Texas Red. Nitrixyte Red can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Red. This kit is optimized for flow cytometry applications.
Size: 100 assays
Description: Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in vivo. It has been challenging to detect and understand the role of NO in biological systems. Fluorimetric Intracellular Nitric Oxide Assay Kit provides a sensitive tool to monitor intracellular NO level in live cells.
Applications: The kit is optimized for flow cytometry applications.
Storage: Keep Component A&B at -20°C and Component C at 4°C.
Kit Components: Component A: Nitrixyte Red (500x) 100 µlComponent B: NONOate Positive Control 1 vial (lyophilized powder)Component C: Assay Buffer 1 bottle (10 ml)
Detection method: Flow cytometer

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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