The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
PARP1: 5 µg; -80°C 5x histone mixture: 1 ml; -80°C 10x Assay Mixture Containing Biotinylated Substrate: 300 µl; -80°C 10x PARP assay buffer: 1 ml; -20°C Blocking buffer: 25 ml; +4°C Activated DNA: 500 µl; -80°C Streptavidin-HRP: 100 µl; +4°C Colorimetric HRP substrate: 10 ml; +4°C Transparent 96-well plate: 1; Room Temp.