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Home > Products > Assay Kits > Activity Kits > PD-1:PD-L2 TR-FRET Assay Kit

PD-1:PD-L2 TR-FRET Assay Kit

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Cat.No.:
Kit-1813
Product Overview:
Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses.
Size:
384 reactions
Description:
The PD-1:PD-L2 TR-FRET Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2 in a homogeneous 384 reaction format. Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L2, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Applications:
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Storage:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
PD-1-Eu: 2 µg; -80°C PD-L2, Biotinylated: 20 µg; -80°C Dye-labeled acceptor: 2 x 10 µl; -20°C 3x PD-1 TR FRET: 4 ml; -20°C White, Nonbinding, low volume, 384-well microtiter plate: 1; Room temp.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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