"PDE4C" Related Products

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PDE4C Assay Kit

Cat.No.: Kit-1840
Product Overview: Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4C selectively hydrolyzes cAMP and is a candidate biomarker for human glioma. Research suggests that PDE4C functions as a tumor suppressor, and downregulation of PDE4C in glioma patients correlates with poor prognosis.
Size: 384 reactions
Description: The PDE4C1 Assay Kit is designed for identification of PDE4C1 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4C1 to the binding agent. The key to the PDE4C1 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4C1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4C1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: PDE4C1 recombinant enzyme: 1 µg; -80°C FAM-Cyclic-3′, 5′-AMP (20 µM): 50 µl; -80°C PDE assay buffer: 25 ml; -20°C Binding Agent: 250 µl; +4°C Binding Agent Diluent: 25 ml; +4°C Black, low binding, microtiter plate: 1; Room temp.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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