"PHF8" Related Products

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PHF8 (KDM7B) Homogeneous Assay Kit

Cat.No.: Kit-1851
Size: 384 rxns
Description: The PHF8 Homogeneous Assay Kit is designed to measure the activity of PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a convenient AlphaLISA format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Applications: Great for studying enzyme kinetics and HTS applications.
Storage: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: PHF8 (KDM7B), (2-525), FLAG-tag: 40 µg; -80°C Primary antibody 17-3: 40 μl; -80°C Biotinylated histone H3 peptide substrate: 500 rxns; -80°C 4x HDM Assay Buffer 3: 2 ml; -20°C 4x HDM Incomplete assay buffer: 1 ml; -20°C 4x Detection buffer: 2 ml; -20°C

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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