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Home > Products > Assay Kits > Activity Kits > PLAT (Human) Chromogenic Activity Assay Kit

PLAT (Human) Chromogenic Activity Assay Kit

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Product Overview:
PLAT (Human) Chromogenic Activity Assay Kit is developed to determine human PLAT activity in cell culture supernatants.
Tissue-type plasminogen activator (tPA) is a 68 kDa serine protease that converts the zymogen plasminogen into the active serine protease plasmin, which digests fibrin and induce the dissolution of fibrin clots. tPA is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo. High level of tPA is a good prognostic marker for breast cancer. tPA may minimize the formation of metastasis by preventing tumor cell adherence at sites of trauma. On the other hand, gastrointestinal cancer is accompanied by a decrease in tPA.
Target Species:
For research use only (RUO)
Upon arrival, immediately store components of the kit at recommended temperatures up to the expiration date.Store Plasminogen, Standard, and Plasmin Substrate at -20°C.Store Microplate and Assay Diluent at 2-8°C. Unused microplate wells may be stored and resealed.
Kit Components:
Microplate: One 96-well polystyrene microplate (12 strips of 8 wells). 96 (8x12) wellsSealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay. 3 slicesAssay Diluent 30 mLtPA Standard: human tPA (32 IU). 1 vialHuman Plasminogen 1 vialPlasmin Substrate 2 vials
Compatible Sample Types:
Cell Culture Supernatant, Plasma

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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