PPAR delta Transcription Factor Assay Kit
| Cat.No. : | Kit-0716 |
| Product Overview : | PPAR delta Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133~10^6 detects human PPAR delta. This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha. |
- Specification
- Gene Information
- Related Products
| Size : | 96 tests |
| Applications : | Functional Studies more details |
Products Types
| ◆ Assay kits | ||
| Kit-0714 | PPAR (alpha, delta, gamma) Transcription Factor Assay Kit | +Inquiry |
| Kit-0717 | PPAR gamma Transcription Factor Assay Kit | +Inquiry |
| Kit-0715 | PPAR alpha Transcription Factor Assay Kit | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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