Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > PRSS7 Assay/Inhibitor Screening Fluorometric Kit

PRSS7 Assay/Inhibitor Screening Fluorometric Kit

   Share
"PRSS7" Related Products.
By Species
Bovine(3)
Mouse(1)
Pig(1)
By Source
By Tag
N/A(3)
Cat.No.:
Kit-0739
Product Overview:
PRSS7 Assay/Inhibitor Screening Kit (Fluorometric)?is used for screening enteropeptidase/enterokinase inhibitors using fluorometric methods.
Description:
Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence "DDDDK" and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition. In PRSS7 Assay/Inhibitor Screening Kit (Fluorometric), we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label "AFC". Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. In the presence of potent Enteropeptidase inhibitor, the hydrolyzation of the substrate will be impeded. The kit provides a rapid, simple & reliable test for screening potential inhibitors of Enteropeptidase.
Applications:
Screening potential inhibitors of EnteropeptidaseCharacterize/study Enteropeptidase inhibitors
Usage:
For research use only (RUO)
Storage:
Store kit at –20°C, protected from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge small vials at low speed (high speed not ideal for enzymes) prior to opening. Read the entire protocol before performing the experiment.
Kit Components:
Enteropeptidase Assay Buffer 20 mlEnteropeptidase Substrate 0.2 mlHuman Enteropeptidase 0.17 mlEnteropeptidase-specific Inhibitor [Aprotinin] (0.6 mM) 0.1 ml
Detection method:
Fluorometric


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.