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Home > Products > Assay Kits > Activity Kits > PTP1B Inhibitor Screening Assay Kit

PTP1B Inhibitor Screening Assay Kit

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By Species
Human(1)
Cat.No.:
Kit-0752
Product Overview:
PTP1B Inhibitor Screening Assay Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity. The kit includes Human, recombinant PTP1B (residues 1-322; MW=37. 4 kDa), expressed in E. coli as a positive control. The PTP1B inhibitor suramin is including as control for inhibitor detection. The detection of free-phosphate released is based on the classic Malachite green assay and offers the advantages of convenient, one-step detection and excellent sensitivity, without radioactivity.
Size:
1 x 96 tests
Applications:
Functional Studies more details
Kit Components:
Components: 1 x 96 tests; 96-well Clear Microplate (1/2 Volume): 1 unit; Phosphate Standard: 1 x 0.5ml; PTP1B Assay Buffer: 1 x 20ml; PTP1B Enzyme (Human, Recombinant): 1 x 5µg; PTP1B Inhibitor: 1 x 10mg; PTP1B Substrate: 1 x 1mg; Red Assay Reagent: 1 x 5ml
Detection method:
Colorimetric
Compatible Sample Types:
Purified protein, Inhibitor compounds


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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