"Peroxidase" Related Products

Peroxidase Activity Assay Kit

Cat.No.: Kit-0675
Product Overview: Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the redfluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (λmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Applications: Functional Studies
Target Species: Reacts with: Human, A wide range of mammals
Storage: Store the kit at-20°C and protect from light. Please read the entire protocol before performing the assay. Avoid repeated freeze/thaw cycles. Warm assay buffer to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components: Components Identifier 100 testsHRP Positive Control Green 1 vialHydrogen Peroxide Substrate Yellow 1 x 0.1mlOxiRed Probe Red 1 x 0.2mlPeroxidase Assay Buffer WM 1 x 25ml
Compatible Sample Types: Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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