Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Detection Kits > Pyrophosphate Fluorometric Assay Kit

Pyrophosphate Fluorometric Assay Kit

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Cat.No.:
Kit-0718
Product Overview:
Pyrophosphates (PPi) are produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters. Pyrophosphate Assay Kit (Fluorometric) provides the most robust spectrophotometric method for the measurement of pyrophosphate. It uses a proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. assay is much easier and more robust than enzyme-coupling pyrophosphate methods, which require at least two enzymes for their pyrophosphate detections. Due to its direct measurement of pyrophosphate, this kit is ideal for screening inhibition or activities of enzymes that consume or generate pyrophosphate. The assay is an optimized mix-and-read assay and can be performed in a convenient 96-well or 384-well microtiter-plate format. The kit provides all the essential components for assaying pyrophosphate.
Size:
2 x 96 tests
Description:
This product measures PPi formation at Ex/Em316/456 nm.If your instrument has filters for Ex/Em370/470 nm, we recommend using Pyrophosphate Assay Kit II (Fluorometric) .
Applications:
Functional Studies more details
Storage:
Store at -20°C.
Kit Components:
Components: 2 x 96 tests; Assay Buffer: 1 x 25ml; DMSO: 1 x 200µl; PPi Sensor: 1 vial; Pyrophosphate Standard (50mM): 1 x 1ml
Compatible Sample Types:
Urine, Serum, Plasma, Cell culture extracts, Adherent cells, Suspension cells, Tissue Lysate


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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