Recombinant canine IL8 (P41324) (Ala23-Pro101) was expressed, with a N-terminal Met.
Predicted N Terminal:
Lyophilized from sterile PBS 0.5mM TCEP pH 7.4, 5%~8% trehalose and mannitol.
The recombinant canine IL8 comprises 80 amino acids and has a predicted molecular mass of 9.2 kDa. The apparent molecular mass of the protein is approximately 9 kDa in SDS-PAGE under reducing conditions due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.