Recombinant Dengue virus Subtype 3 Envelope III domain immunodeterminant regions(aa 300-400) fused with His tag was expressed in E. coli.
Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus.
Phosphate buffered saline, pH-7.4 and 50mM arginine.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Each laboratory should determine an optimum working titer for use in its particular application.
Dengue Envelope-ST3 D-III although stable at 4 centigrade for 1 week, should be stored below -18 centigrade. Please prevent freeze thaw cycles.