Recombinant Dengue virus 2 Envelope(aa 298-400) fused with His tag at the N-terminus was expressed in E. coli.
Envelope protein E takes part in membrane fusion between virion and host late endosomes. Virus Envelope 2 is synthesized as a homodimer with prM which plays a role as a chaperone for envelope protein E. Envelope protein E departs from small envelope protein M and homodimerizes after cleavage of prM.
The Dengue Envelope 2 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
MGSSHHHHHH SSGLVPRGSH MGSSYSMCTG KFKVVKEIAE TQHGTIVIRV QYEGDGSPCK IPFEIMDLEK RHVLGRLITV NPIVTEKDSP VNIEAEPPFG DSYIIIGVEP GQLKLNWFKK GSSIGQ.
Greater than 95% as determined by SDS-PAGE.
Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.