Recombinant Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) NP protein(His630-Gln739), His-tagged

• Cat.No.: NP-802V
• Product Overview: Recombinant Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) NP (AHX24646.1) (His630-Gln739) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: EBOV
• Tag: N-His
• Protein length: His630-Gln739
• Form: Lyophilized from sterile 50 mM Tris, 500 mM NaCl, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) NP consists 128 amino acids and predicts a molecular mass of 15.6 kDa.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.