Recombinant Ferret (Mustela putorius furo) CD8A (ABS50091.1) extracellular domain (Met 1-Glu 186) was expressed, with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA. 2. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA. 3. Measured by its ability to bind biotinylated recombinant hum
The recombinant Ferret CD8A consists of 175 amino acids and has a calculated molecular mass of 19.5 kDa. The apparent molecular mass of the recombinant protein is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>92 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.