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Home > Products > Recombinant Proteins > Recombinant H5N1 (A/Anhui/1/2005) HA Protein, His-tagged

Recombinant H5N1 (A/Anhui/1/2005) HA Protein, His-tagged

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By Tag
Fc(4)
Fc-His(1)
His(359)
His & Fc(1)
Cat.No.:
HA-274V
Product Overview:
Recombinant extracellular domain of Influenza A virus (A/Anhui/1/2005 (H5N1) (ABD28180.1) hemagglutinin (Met 1-Gln 527) with cleavage site mutated (RERRRKR→TETR, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag.
Source:
HEK293
Species:
H5N1
Tag:
His
Predicted N Terminal:
Asp 17
Form:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass:
The secreted recombinant hemagglutinin of Influenza A virus (A/Anhui/1/2005 (H5N1) comprises 522 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
>97 % as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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