Recombinant extracellular domain of Influenza A virus (A/bar-headed goose/Qinghai/14/2008 (H5N1)) (ACL28277.1) HA (Met 1-Gln 531) with cleavage site mutated (RERRRKKR →TETR,HA1+HA2, uncleaved) was expressed, with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 5%~8% trehalose and mannitol.
The recombinant hemagglutinin of Influenza A virus (A/bar-headed goose/Qinghai/14/2008 (H5N1)) comprises 522 amino acids and has a calculated molecular mass of 59.5 kDa as estimated in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>85 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.