Recombinant extracellular domain of Influenza A virus H9N2 hemagglutinin (Influenza A virus (A/Hong Kong/1073/99 (H9N2)) (NP_859037.1) (Met 1-Lys 523) (HA1+HA2, uncleaved) was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
The secreted recombinant hemagglutinin of Influenza A virus H9N2 (A/Hong Kong/1073/99) comprises 516 amino acids with the predicted molecular mass of 58 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.