Recombinant HPV16 L1 fused with His tag was expressed in E. coli.
It is supplied in 50 mM Tris-HCl (pH 8), 0.25M NaCl, 5 mM b-ME, 0.25M Imidazole, and 8M Urea (lot sp. conc on the vial).
Western Blotting. Purified protein should be tested at 50-500 ng/lane and reacted with appropriate antibodies.ELISA: Control protein should be coated at 1-10 ug/ml.
Store at -20 centigrade or below for at least 6 months.
Short-term: unopened, undiluted vials for less than a week at 4 centigrade.Long-term: at – 20 centigrade or below in suitable aliquots after reconstitution. Do not freeze and thaw and store working, diluted solutions.
4 centigrade for solutions and room temp for powder.