The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci.
Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
1 mg/ml in PBS (-), 1 mM DTT, 50% glycerol, sterilized by filtration
The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo.
Over 98% by SDS-PAGE
Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37centigrade for 30 min.
1).Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent).
2).Reagent for membrane pore formation to introduce small-to-macromolecules into living cells.
Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT.
Store at -20 centigrade(long period, -70 centigrade).
Handling and storage precautions: Biohazard. Do not use if skin is cut or scratched.
Other precautions: Substance not yet fully tested.
Protective gloves: compatible chemical-resistant gloves.
Eye protection: ansi approved chemical workers goggle.
Other protective equipment: eye wash and deluge shower meeting ansi design criteria. Work hygienic practices: wash thoroughly after handling.