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Home > Products > Recombinant Proteins > Recombinant Hemolytic streptococcus Streptolysin O

Recombinant Hemolytic streptococcus Streptolysin O

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Cat.No.:
slo-001S
Product Overview:
The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci.
Description:
Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
Source:
E. coli
Species:
Hemolytic streptococcus
Form:
1 mg/ml in PBS (-), 1 mM DTT, 50% glycerol, sterilized by filtration
Bio-activity:
The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo.
AA Sequence:
NKQNTANTETTTTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDNKKSEEDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTPVDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVNDPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTLKELQRKGVSNEAPPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRSEYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYGSITYK
Purity:
Over 98% by SDS-PAGE
Unit Definition:
Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37centigrade for 30 min.
Applications:
1).Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent).
2).Reagent for membrane pore formation to introduce small-to-macromolecules into living cells.
Notes:
Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT.
Storage:
Store at -20 centigrade(long period, -70 centigrade).
Warning:
Handling and storage precautions: Biohazard. Do not use if skin is cut or scratched.
Other precautions: Substance not yet fully tested.
Protective gloves: compatible chemical-resistant gloves.
Eye protection: ansi approved chemical workers goggle.
Other protective equipment: eye wash and deluge shower meeting ansi design criteria. Work hygienic practices: wash thoroughly after handling.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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