Active Recombinant Human ACHE protein, His-tagged

• Cat.No.: ACHE-105H
• Product Overview: Recombinant Human ACHE protein(Glu 32 - Leu 614), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave Acetylthiocholine. The specific activity is >500 nmol/min/μg, as measured under the described conditions.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 66.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Glu 32 - Leu 614
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACHE acetylcholinesterase [ Homo sapiens ]
• Official Symbol: ACHE
• Synonyms: ACHE; acetylcholinesterase; acetylcholinesterase (YT blood group) , acetylcholinesterase (Yt blood group) , YT; Yt blood group; apoptosis-related acetylcholinesterase; YT; ACEE; ARACHE; N-ACHE
• Gene ID: 43
• mRNA Refseq: NM_000665
• Protein Refseq: NP_000656
• MIM: 100740
• UniProt ID: P22303

Reviews

12/12/2020

I have already recommended creative biomart products to many colleagues.

05/09/2019

Received the product very quickly after placing the order, I am very satisfied.

Q&As

When can I receive the product after ordering?

The time required for different proteins varies, you can pay attention to the information on the product details page, or contact our experts directly.

Can ACHE-3333HFL products change tags?

Sure. We can customize proteins according to customer needs, including different tags, expression system, protein length, etc.

Why the protein length of ACHE-101H product is 1-351aa?

Because we can customize proteins of different lengths according to customer needs, please contact us if you are interested in this product.

What is the mechanism by which patients with Alzheimer's disease are treated with acetylcholinesterase inhibitors?

Patients with Alzheimer disease often receive treatment with acetylcholinesterase inhibitors that mitigate symptoms by hindering ACh turnover.

In which tissues is acetylcholinesterase mainly distributed?

Acetylcholinesterase is known to be distributed in nervous tissue such as the brainstem, cerebellum, and peripheral and autonomic nervous systems. Skeletal muscle also contains AChE with distribution patterns seemingly related to the type of muscle (fast versus slow twitch) and their specific function. AChE is also present on red blood cell membranes.

Does ACHE only work on terminating neurotransmission?

Although the primary function of AChE is to terminate neural transmission, investigators have found that AChE also plays a role in neural development. During fetal development, AChE contributes to morphogenesis in addition to its main enzymatic function.

What form does acetylcholinesterase usually exist in?

Acetylcholinesterase exists as a monomer that often polymerizes into a dimer with a disulfide bond. Along with Van der Waals forces, two dimers may be linked to become tetramers.

What is the role of ACHE?

The primary role of AChE is to terminate neuronal transmission and signaling between synapses to prevent ACh dispersal and activation of nearby receptors. They are an important component of pesticides and nerve agents.