Recombinant Human ACHE(Glu32-Leu614) fused with His tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Measured by its ability to cleave Acetylthiocholine. The specific activity is >500 nmol/min/μg.
Predicted Molecular Mass: 65 kDa;SDS-PAGE: 60-75 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.