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Home > Products > Recombinant Proteins > Recombinant Human ANOS1 protein, His-tagged

Recombinant Human ANOS1 protein, His-tagged

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By Species
Human(1)
By Source
CHO(1)
By Tag
His(1)
Cat.No.:
ANOS1-206H
Product Overview:
Recombinant Human ANOS1(Ala25-Tyr680) fused with His tag at C-terminal was expressed in CHO.
Description:
Anosmin-1, also known as Kal-1, is a 100 kDa secreted glycoprotein that regulates neuronal adhesion and migration. Mutations in Anosmin-1 are associated with the development of X-linked Kallman syndrome which can be accompanied by hearing loss. Mature human Anosmin-1 contains one WAP domain and four Fibronectin type-III domains. It is expressed by cerebellar Purkinje cells and deep nuclear neurons, piriform cortex and olfactory bulb mitral cells, optic tectum and retinal ganglion axons, and oligodendrocyte progenitor cells (OPC). Anosmin-1 binds to Integrin beta 1/CD29, FGF R1, L1CAM, and complexes of FGF R1 with FGF basic and heparan sulfate proteoglycans, and it enhances signaling through FGF R1. It mediates OPC adhesion, inhibits FGF basic-induced OPC migration, and enhances oligodendrocyte-mediated axon myelination. It also cooperates with FGF basic to promote olfactory axon elongation and neurite outgrowth. In addition, Anosmin-1 is up-regulated in glioblastoma and gastric cancer where it enhances tumor cell proliferation and migration.
Source:
CHO
Species:
Human
Tag :
His
Predicted N Terminal:
Ala25
Form:
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
Bio-activity:
Measured by its binding ability in a functional ELISA. When Recombinant Human FGF basic 146 aa Recombinant Human FGF basic 146 aa is coated at 1.5 µg/mL, 100 μL/well, Recombinant Human KAL1 binds with an ED50 of 8-48 ng/mL.
Molecular Mass:
Predicted Molecular Mass: 75 kDa
SDS-PAGE: 84-103 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 200 μg/mL in water.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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