Recombinant Human APLF protein, DDK-tagged

• Cat.No.: APLF-12H
• Product Overview: Recombinant Human APLF fused with DDK tag at C-terminal was expressed in Insect cells.

Specification

• Source: Insect cells
• Species: Human
• Tag: DDK
• Form: 50mM Tris-HCl, pH8.0, 100mM glycine, 10% glycerol.
• Molecular Mass: 56.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: APLF aprataxin and PNKP like factor [ Homo sapiens ]
• Official Symbol: APLF
• Synonyms: APLF; aprataxin and PNKP like factor; C2orf13, chromosome 2 open reading frame 13; aprataxin and PNK-like factor; MGC47799; XRCC1-interacting protein 1; PNK and APTX-like FHA protein; aprataxin- and PNK-like factor; apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; APFL; PALF; Xip1; C2orf13; FLJ16593
• Gene ID: 200558
• mRNA Refseq: NM_173545
• Protein Refseq: NP_775816
• MIM: 611035
• UniProt ID: Q8IW19
• Chromosome Location: 2p14
• Function: 3-5 exonuclease activity; DNA-(apurinic or apyrimidinic site) lyase activity; endodeoxyribonuclease activity; lyase activity; metal ion binding; nucleotide binding; protein binding

Reviews

07/01/2022

The strip shape of APLF in Western blot is regular, indicating that it has excellent uniformity and predictability.

04/06/2021

After using APLF, the sensitivity of the experiment was significantly improved.

03/29/2021

The production process of APLF fully reflects the concept of green and environmental protection and contributes to sustainable development.

01/03/2020

It can maintain stable performance even after multiple uses, which makes me more confident in the experimental results.

Q&As

What role does recombinant APLF play in the treatment of immunodeficiency disease?

APLF may play a role in adjuvant therapy or targeted therapy in the treatment of immune deficiency diseases.

What is the expression of APLF in cancer cells?

APLF may be expressed differently in some cancer cells.

What is the mechanism of action of APLF in various cancers?

APLF may play different mechanisms of action in different cancers, and further research is needed.

What is the relationship between APLF and DNA single-strand structure?

APLF is a reaction factor for the single strand structure of DNA.

What are the different expression levels of APLF in different tissues?

The expression level of APLF is different in different tissues.

What is the relationship between APLF and DNA repair?

APLF is an important factor in the repair process of DNA double-strand breaks after DNA damage.

What role does APLF play in cancer treatment?

APLF may play a role in adjuvant therapy or targeted therapy in cancer therapy.

What is the effect of APLF on DNA single strand cut repair?

APLF may play an important role in the process of DNA single strand cleavage repair.

What is the effect of APLF on DNA damage repair?

Recombinant APLF proteins may affect the efficiency or manner in which DNA damage is repaired.

What role does recombinant APLF play in tumor immunotherapy?

APLF may play a role in adjuvant therapy or targeted therapy in tumor immunotherapy.

How to prepare APLF ?

APLF can be prepared by genetic engineering and other technologies.

What is the functional mechanism of APLF ?

The functional mechanism of APLF includes interaction with other molecules.

What is the function of APLF ?

APLF plays an important role in DNA repair, apoptosis, cell cycle and other processes.

What role does APLF play in tumor prognosis?

APLF may be a potential prognostic marker in tumor prognosis and needs to be further validated.

Is APLF helpful for drug therapy?

APLF may have synergistic effects on drug therapy.

What are the signaling pathways associated with APLF and tumor?

APLF is related to signal transduction pathways such as PI3K-Akt signaling pathway and NF-κB signaling pathway.

Which diseases are APLF s associated with?

APLF is associated with certain cancers, immune deficiency diseases and other diseases.

What are the structural characteristics of APLF ?

The structural characteristics of APLF s include the presence of nucleotide binding sites.

What is the role of APLF in apoptosis?

APLF may play an important role in cell apoptosis.