Recombinant Human APOC3 protein, His/GST-tagged

• Cat.No.: APOC3-393H
• Product Overview: Recombinant Human APOC3(Ser21~Ala99) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Description: Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in ap centigrade-III levels induces the development of hypertriglyceridemia.
• Source: E. coli
• Species: Human
• Tag: His/GST
• Form: Supplied as lyophilized form in 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Molecular Mass: 40.7kDa
• Protein length: Ser21~Ala99
• AA Sequence: SEAEDASLLS FMQGYMKHAT KTAKDALSSV QESQVAQQAR GWVTDGFSSL KDYWSTVKDK FSEFWDLDPE VRPTSAVAA
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: APOC3 apolipoprotein C-III [ Homo sapiens ]
• Official Symbol: APOC3
• Synonyms: APOC3; apolipoprotein C-III; apo-CIII; ap centigrade-III; apolipoprotein C3; HALP2; APOCIII; MGC150353
• Gene ID: 345
• mRNA Refseq: NM_000040
• Protein Refseq: NP_000031
• MIM: 107720
• UniProt ID: P02656
• Chromosome Location: 11q23-qter
• Pathway: Chylomicron-mediated lipid transport, organism-specific biosystem; HDL-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem
• Function: cholesterol binding; enzyme regulator activity; high-density lipoprotein particle receptor binding; lipase inhibitor activity; phospholipid binding