Recombinant human APOH (NP_000033.2) (Met 1-Cys 345) was expressed, with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA.2. Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated human LDLR-his, The EC50 of biotinylated human LDLR-his is 0.35 μg/mL.3.Immobilized human APOH-his at 2 μg/mL (100 μl/we
The recombinant human APOH consists of 337 amino acids and predictes a molecular mass of 37.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhAPOH is approximately 53 kDa due to different glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>96 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.