| Product overview : |
Recombinant Human APRT (P07741) aa. (Met1~Glu180) fused with His tag was produced in E. coli cells. |
| Description : |
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
Predicted Molecular Mass: 23.3kDa; Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
Met1~Glu180 |
| Purity : |
>98% |
| Characteristic : |
Isoelectric point is 6.8. |
| Applications : |
Cell culture; Activity Assays; In vivo assays. |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Storage buffer : |
20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
