Recombinant Human AurB [1-344(end) amino acids of accession number Q96GD4] fused with DYKDDDDK tag at N-terminal and biotinylated was co-expressed with His-tagged INCENP(INBOX) [803-918(end) amino acids of accession number AAU04398] in insect cell.
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
>82% as determined by SDS-PAGE and CBB staining
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.