Recombinant Human BMP15(Thr266~Ser388) fused with His tag at N-terminal was expressed in E. coli.
Predicted N Terminal:
TRQAD GISAEVTASS SKHSGPENNQ CSLHPFQISF RQLGWDHWII APPFYTPNYC KGTCLRCLRD GLNSPNHAII QNLINQLVDQ SVPRPSCVPY KYVPISVLMI EANGSILYKE YEGMIAES
100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Predicted Molecular Mass: 17.6kDa;Accurate Molecular Mass: 18kDa(Analysis of differences refer to the manual).
<1.0EU per 1µg (determined by the LAL method)
SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.