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Home > Products > Recombinant Proteins > Recombinant Human CCNE1 Protein, His & GST-tagged

Recombinant Human CCNE1 Protein, His & GST-tagged

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By Species
Chicken(1)
Human(13)
Mouse(6)
Rat(3)
Zebrafish(1)
By Tag
GST(1)
His(4)
His & GST(1)
None(1)
Cat.No.:
CCNE1-259H
Product Overview:
Recombinant full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source:
Baculovirus-Insect Cells
Species:
Human
Tag:
His & GST
Predicted N Terminal:
Met
Form:
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, 1mM GSH, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity:
Measured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.
Molecular Mass:
The recombinant human CCNE1/GST chimera consists of 647 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
>82 % as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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