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Recombinant Human CD47 protein, Fc/Avi-tagged, Biotinylated

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Cat.No.:
CD47-7890H
Product Overview:
Biotinylated Recombinant Human CD47 (Gln 19 - Pro 139) fused with human IgG1 Fc fragment at the C-terminus, followed by an Avi tag was expressed in HEK293.
Source:
HEK293
Species:
Human
Tag:
Fc/Avi, Biotinylated
Predicted N Terminal:
Gln 19
Form:
Lyophilized from 0.22µm filtered solution in 50 mM tris, 100 mM glycine (pH7.5) with 10% Trehalose as protectant.
Bio-activity:
Immobilized Human SIRP alpha Protein, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47 Protein, Fc Tag with a linear range of 8-250 ng/mL.
Molecular Mass:
The protein has a calculated MW of 42.2 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Protein length:
Gln 19 - Pro 139
Endotoxin:
Less than 1.0 EU per μg by the LAL method.
Purity:
>95% as determined by SDS-PAGE.
Storage:
For long term storage, the product should be stored at lyophilized state at -20 centigradeor lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigradefor 12 months in lyophilized state;-70 centigradefor 3 months under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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