Biotinylated Recombinant Human CD47 (Gln 19 - Pro 139) fused with human IgG1 Fc fragment at the C-terminus, followed by an Avi tag was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22µm filtered solution in 50 mM tris, 100 mM glycine (pH7.5) with 10% Trehalose as protectant.
Immobilized Human SIRP alpha Protein, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47 Protein, Fc Tag with a linear range of 8-250 ng/mL.
The protein has a calculated MW of 42.2 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Gln 19 - Pro 139
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
For long term storage, the product should be stored at lyophilized state at -20 centigradeor lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigradefor 12 months in lyophilized state;-70 centigradefor 3 months under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.