Recombinant Human CDH1 protein, His-tagged

• Cat.No.: CDH1-691H
• Product Overview: Recombinant Human CDH1(Gln 23 - Phe 647) fused with His tag at C-terminal was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4
• Protein length: Gln23-Phe647
• AA Sequence: QEPEPCHPGFDAESYTFTVPRRHLE RGRVLGRVNFEDCTGRQRTAYFSLD TRFKVGTDGVITVKR PLRFHNPQIHFLVYAWDSTYRKFST KVTLNTVGHHHRPPPHQASVSGIQA ELLTFPNSSPGLRRQ KRDWVIPPISCPENEKGPFPKNLVQ IKSNKDKEGKVFYSITGQGADTPPV GVFIIERETGWLKVT EPLDRERIATYTLFSHAVSSNGNAV EDPMEILITVTDQNDNKPEFTQEVF KGSVMEGALPGTSVM EVTATDADDDVNTYNAAIAYTILSQ DPELPDKNMFTINRNTGVISVVTTG LDRESFPAYTLVVQA ADLQGEGLSTTATAVITVTDTNDNP PIFNPTTYKGQVPENEANVVITTLK VTDADAPNTPAWEAV YTILNDDGGQFVVTTNPVNNDGILK TAKGLDFEAKQQYILHVAVTNVVPF EVSLTTSTATVTVDV LDVNEAPIFVPPEKRVEVSEDFGVG QEITSYTAQEPDTFMEQKITYRIWR DTANWLEINPDTGAI STRAELDREDFEHVKNSTYTALIIA TDNGSPVATGTGTLLLILSDVNDNA PIPEPRTIFFCERNP KPQVINIIDADLPPILASQSIGITD MSHCTCPAPQLPAIFVDHHHHHH*
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.Aliquots of reconstituted samples are stable at < -20 centigrade for 4 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in 2X PBS.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CDH1 cadherin 1, type 1, E-cadherin (epithelial) [ Homo sapiens ]
• Official Symbol: CDH1
• Synonyms: CDH1; cadherin 1, type 1, E-cadherin (epithelial); UVO; cadherin-1; CD324; E Cadherin; uvomorulin; CAM 120/80; E-Cadherin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; CDHE; ECAD; LCAM; Arc-1
• Gene ID: 999
• mRNA Refseq: NM_004360
• Protein Refseq: NP_004351
• MIM: 192090
• UniProt ID: P12830
• Chromosome Location: 16q22.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesionproteins, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem
• Function: GTPase activating protein binding; calcium ion binding; cell adhesion molecule binding; gamma-catenin binding; protein binding

Reviews

04/22/2022

This recombinant protein has a very short half-life and shows a very high clearance rate, which makes its biological activity more stable.

05/27/2020

The protein was highly purified and was not doped with other proteins or impurities.

05/21/2019

CDH1 has similar activity to the native protein and is able to perform the expected biological functions.

Q&As

How about the inheritance pattern of CDH1 gene mutations?

CDH1 gene mutations are usually passed on in an autosomal dominant manner, depending on the type and location of the mutation.

What screening is required for carriers of CDH1 gene mutations?

Carriers of CDH1 gene mutations should have gastroscopy, ultrasonography, and other related tests to monitor the development and progression of stomach cancer.

Can CDH1 gene mutations be prevented or treated?

For people with mutations in the CDH1 gene, prophylactic total gastrectomy may reduce the risk of diffuse stomach cancer. There is currently no specific treatment to target CDH1 gene mutations.

Which gastric cancer subtypes are associated with CDH1 mutations?

CDH1 gene mutation is closely related to the occurrence of diffuse gastric cancer, which is a special subtype of gastric cancer with a high degree of invasion and malignancy.

What are the effects of CDH1 gene mutations on cell adhesion and tissue differentiation?

The CDH1 gene encodes a protein called E-calcin that plays an important role in cell adhesion and tissue differentiation. Mutations in the CDH1 gene may affect these processes, resulting in abnormal cell adhesion and tissue differentiation.

What is the relationship between CDH1 gene mutations and breast cancer?

CDH1 gene mutations may be associated with the development of a small number of breast cancers, because CDH1 also plays a role in regulating cell adhesion and tissue structure in breast tissue.